Membrane receptor proteins are key elements in the interactions between cells and their environment. Upon binding their cognate ligands, receptors initiate a cascade of signaling events inside the cell, which ultimately determine a final cell response. In order to analyze these signaling events as a function of the spatial arrangement of ligands, interdisciplinary technology solutions are required, which enable control over number, stoichiometry, and precise nanoscale orientation of ligands. We apply DNA origami-based nanostructures to create surface-bound ligand architectures that comprise a lateral resolution of different ligands with distances in the typical range of biomolecular assemblies (~5-100 nm).[1] In this project we strive to use this technology for the investigation of receptor activation and recruitment of downstream signaling components in mast cells.