Function and localization of membrane-active peptides by NMR and MRI
- Project Group:
Project are B
Membrane-active peptides or proteins, which are responsible for the transport of material and information across membranes, are crucial for drug delivery. To address the issue of function and localization of membrane-active peptides, the biophysical methods of fluorescence and NMR spectroscopy could be combined, the already well- established fluorescence imaging in transparent zebrafish embryos are used to develop novel 19F-MRI imaging methods that can then be applied to non-transparent organisms.
In view of no 19F background signal in the soft tissue of human as well as its inertia of perfluorocarbon, PFC, as well as nanoparticles (fluorescent and 19F-containing) as fluoro-tracer are used to investigate and compare fish mutants that show vascularization disorders. Bioactive cell-penetrating peptides from project B4 are additionally labeled with fluorescent probes (or their intrinsic fluorescence is observed, photocontrollable cell-penetrating peptides based on diarylethene amino acids) in order to correlate this with 19F-MRI on 19F-labeled peptides. Dual-labeled Peptides can be applied to zebrafish embryos for localization of the amyloid pathogens of neurodegenerative diseases and localization of signal transmission processes.